Bộ phim xoay quanh câu chuyện về những con người nặng lòng với nghệ thuật cải lương cùng những nỗi truân chuyên, đoạn trường nam ai của nghiệp cầm ca, đồng thời đưa khán giả có cơ hội khám phá những hình ảnh mang đậm nét sinh hoạt văn hóa vùng đất phương Nam.
Kể lại câu chuyện Đoạn Trường Nam Ai là bà già nấu cháo từ thiện. Ngày xưa, bà là đào hát, có tên cô Ba Tuyết Sương. Lúc đó, khi cô còn tên là Mận, cô có hai người bạn thân là Hai Hoài và Tố Phụng gắn bó từ nhỏ tới khi trưởng thành ở vùng Bạc Liêu. Mận có năng khiếu hát ca, còn Phụng diễn giỏi nhưng ca không được. Hai Hoài có tài thổi sáo và thẩm âm giỏi nên được ông thầy đờn Chín Kìm truyền nghề. Và câu chuyện tiếp nối đến 15 năm sau, khi thế hệ con cái của họ tiếp nối nghiệp cầm ca.
Diễn viên: Bạch Lan, Hoài Linh, Xuân Hương; Minh Phượng, Tuyết Thu, Bảo Châu, Hoài Lâm, Lý Hùng, Hà Trí Quang, Thanh Trúc, Bùi Lê Kim Ngọc, Quốc Trầm, Lâm Mỹ Vân, Võ Hiệp, Dương Mỹ Dung, Kiều Khanh, Trọng Nhân…
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